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KMID : 1007020110090010066
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Korean Soceity of Osteroporosis
2011 Volume.9 No. 1 p.66 ~ p.79
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The Relationship between Polymorphisms in Tumor Necrosis Factor (TNF) Genes and Changes in Bone Mineral Density after Hormone Therapy in Postmenopausal Korean Women
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Chun Sung-Wook
Kim Jung-Gu
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Abstract
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Objectives: To determine the association between polymorphisms in tumor necrosis factor-α (TNF-α), and TNF-β genes, and changes in bone mineral density (BMD) after hormone therapy (HT) in postmenopausal Korean women.
Materials & Methods: The TNF-α G(-308)A, C(-857)T, C(-863)A, T(-1031)C, and TNF-β A252G polymorphisms were analyzed by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) or Taqman assay in 218 postmenopausal Korean women receiving sequential HT for 1 year. Bone mineral density (BMD) at the lumbar spine and femoral neck was examined by dual energy X-ray absorptiometry. Serum levels of bone specific alkaline phosphatase (BAP), osteocalcin (OST), type I C-telopeptide breakdown products (CTX), parathyroid hormone (PTH), calcium, and phosphorus were measured using enzyme-linked immunosorbent assay (ELISA), immunoassay, and atomic absorptiometry, respectively.
Results: No significant associations were found between single genotypes of TNF-α, or TNF-β polymorphisms and the annual percentage changes of BMD at the lumbar spine and femoral neck after HT. Haplotype analysis of 4 TNF-α polymorphisms did not show any significant association with the annual percentage changes of BMD at the lumbar spine and femoral neck after HT. The changes in serum levels of bone markers 6 months after HT were not related with single genotypes or haplotype genotypes of TNF polymorphisms. The distribution of these single or haplotype genotypes were not different between HT responders and HT non-responders (women who lose >3% of bone mass per year).
Conclusions: The TNF-α G(-308)A, C(-857)T, C(-863)A, T(-1031)C, and TNF-β A252G polymorphisms are not associated with changes in BMD after HT in postmenopausal Korean women.
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KEYWORD
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Korean postmenopausal women, TNF, Gene polymorphism, Hormone therapy, BMD
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